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Anti-inflammatory activity of Seabuckthorn (Hippophae
rhamnoides) leaves.
Defence Institute of Physiology and Allied
Sciences, Lucknow Road, Timarpur, Delhi-110054, India.
lganju@rediffmail.com
Immunomodulatory activity of Seabuckthorn (SBT)
leaf extract was evaluated in adjuvant induced arthritis (AIA) rat
model. Inflammation was induced by injecting Complete Freund's
Adjuvant (CFA) in the right hind paw of rats. SBT extract was
administered intraperitoneally to treat the inflammation. The
extent of inflammation and treatment response was evaluated by
clinical analysis, scintigraphic visualization using
technitium-99m-glutathione (Tc99m-GSH) and lymphocyte
proliferation. Serial evaluation was carried out on days 1, 7, 14,
21 and 28 after creation of inflammation. The Tc99m-GSH uptake in
the inflamed leg was compared with the normal contralateral leg of
the same animal. The measurements were done by obtaining
scintigraphic images using gamma camera and an online computer.
Both qualitative and quantitative evaluation of radiotracer
accumulation was considered to evaluate the anti-inflammatory
response. The lymphocyte proliferation study revealed cellular
immunosuppression during the early phase of the disease.
Administration of SBT extract on the same day or 5 days prior to
inflammatory insult into the joint, significantly reduced the
inflammation as compared to the untreated animals in a dose
dependent manner. These observations suggest that the SBT leaf
extract has a significant anti-inflammatory activity and has the
potential for the treatment of arthritis.
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